Study: The Effect of Probiotic Cleaning Methods on Mold Reduction

A scientific study conducted in a school in Tallinn demonstrated the remarkable effectiveness of probiotic cleaning methods in controlling mold. The study was carried out over a 26-day period, focusing on three adjacent rooms: a toilet, a washroom, and a corridor.

Study Methodology

The following protocol was used during the study:

• A 3% solution of Probiotic Universal Cleaner
• Cleaning frequency: 2 times per week
• Testing period: 26 days
• Surface samples were taken from three designated points

Measured Results

The following changes in mold occurrence were documented during the study (CFU — Colony Forming Unit):

Toilet Floor Corner

• Initial level (11.11.2021): Uncountable growth
• Intermediate level (26.11.2021): 50 CFU
• Final level (07.12.2021): 50 CFU

Washroom Wall Corner

• Initial level: 4,700 CFU
• Intermediate level: 53 CFU
• Final level: 30 CFU

Corridor Floor Corner

• Initial level: 3,500 CFU
• Intermediate level: 0 CFU
• Final level: 0 CFU

Scientific Conclusions

The study results show a statistically significant reduction in mold on all tested surfaces. Notably:

1. Probiotic spore bacteria successfully colonized the treated surfaces
2. Mold reduction was noticeable already by the middle of the test period
3. The effect remained stable throughout the entire test period

Limitations and Observations

It is important to note that 100% effectiveness was not achieved, which can be explained by the continuous migration of mold spores from adjacent rooms. This observation is important for planning further studies and developing practical applications.

Practical Significance

The study demonstrates that the use of probiotic cleaning methods:

• Significantly reduces mold occurrence on surfaces
• Allows for a reduction in cleaning frequency
• Provides a sustainable solution for keeping mold under control

Conclusion

The study results confirm the effectiveness of probiotic cleaning methods in reducing mold in a school environment. This provides a science-based foundation for the use of probiotic cleaning products in public institutions where mold control is of critical importance.